Journal: Microorganisms

Article Title: In-Feed vs. In-Water Chlortetracycline Administration on the Fecal Prevalence of Virulence Genes and Pathotypes of Escherichia coli Involved in Enteric Colibacillosis in Piglets

doi: 10.3390/microorganisms13061185

Figure Lengend Snippet: QIAxcel image of the eleven virulence genes of enteric pathotypes of Escherichia coli implicated in swine colibacillosis.

Article Snippet: Approximately 1 g of fecal sample was suspended in 9 mL of Escherichia coli (EC) broth (Becton Dickinson and Co., Sparks, MD, USA) and vortexed for 1 min; the fecal suspension was incubated at 40 °C for 6 h. After incubation, 1 mL of the enriched fecal suspension was pipetted into a 2 mL centrifuge tube and subjected to DNA extraction [ ].

Techniques:

Journal: Microorganisms

Article Title: In-Feed vs. In-Water Chlortetracycline Administration on the Fecal Prevalence of Virulence Genes and Pathotypes of Escherichia coli Involved in Enteric Colibacillosis in Piglets

doi: 10.3390/microorganisms13061185

Figure Lengend Snippet: Overall sample level-based prevalence of the major virulence genes of Escherichia coli pathotypes involved in swine colibacillosis in fecal samples collected from piglets administered with or without in-feed or in-water chlortetracycline.

Article Snippet: Approximately 1 g of fecal sample was suspended in 9 mL of Escherichia coli (EC) broth (Becton Dickinson and Co., Sparks, MD, USA) and vortexed for 1 min; the fecal suspension was incubated at 40 °C for 6 h. After incubation, 1 mL of the enriched fecal suspension was pipetted into a 2 mL centrifuge tube and subjected to DNA extraction [ ].

Techniques:

Journal: Microorganisms

Article Title: In-Feed vs. In-Water Chlortetracycline Administration on the Fecal Prevalence of Virulence Genes and Pathotypes of Escherichia coli Involved in Enteric Colibacillosis in Piglets

doi: 10.3390/microorganisms13061185

Figure Lengend Snippet: Overall sample level-based prevalence of Escherichia coli isolates positive for one or more of the virulence genes involved in swine colibacillosis in fecal samples collected from piglets administered with or without in-feed or in-water chlortetracycline.

Article Snippet: Approximately 1 g of fecal sample was suspended in 9 mL of Escherichia coli (EC) broth (Becton Dickinson and Co., Sparks, MD, USA) and vortexed for 1 min; the fecal suspension was incubated at 40 °C for 6 h. After incubation, 1 mL of the enriched fecal suspension was pipetted into a 2 mL centrifuge tube and subjected to DNA extraction [ ].

Techniques:

Journal: Microorganisms

Article Title: In-Feed vs. In-Water Chlortetracycline Administration on the Fecal Prevalence of Virulence Genes and Pathotypes of Escherichia coli Involved in Enteric Colibacillosis in Piglets

doi: 10.3390/microorganisms13061185

Figure Lengend Snippet: Overall sample level-based prevalence of Escherichia coli pathotypes involved in swine colibacillosis in fecal samples collected from piglets administered with or without in-feed or in-water chlortetracycline.

Article Snippet: Approximately 1 g of fecal sample was suspended in 9 mL of Escherichia coli (EC) broth (Becton Dickinson and Co., Sparks, MD, USA) and vortexed for 1 min; the fecal suspension was incubated at 40 °C for 6 h. After incubation, 1 mL of the enriched fecal suspension was pipetted into a 2 mL centrifuge tube and subjected to DNA extraction [ ].

Techniques:

Journal: Microorganisms

Article Title: In-Feed vs. In-Water Chlortetracycline Administration on the Fecal Prevalence of Virulence Genes and Pathotypes of Escherichia coli Involved in Enteric Colibacillosis in Piglets

doi: 10.3390/microorganisms13061185

Figure Lengend Snippet: Overall sample level-based prevalence of enterotoxigenic Escherichia coli in fecal samples collected from piglets administered with or without in-feed or in-water chlortetracycline.

Article Snippet: Approximately 1 g of fecal sample was suspended in 9 mL of Escherichia coli (EC) broth (Becton Dickinson and Co., Sparks, MD, USA) and vortexed for 1 min; the fecal suspension was incubated at 40 °C for 6 h. After incubation, 1 mL of the enriched fecal suspension was pipetted into a 2 mL centrifuge tube and subjected to DNA extraction [ ].

Techniques: